The 2024 finalists, the Arthurian League and the West Yorkshire Association League, are exempt until the Quarter-Finals
| First Round (by 7 February) | ||||||||
| 07.Feb | Liverpool FA Premier League |  | vs |  | Manchester League | — | at Liverpool FA, Sefton | |
| 31.Jan | York League |  | vs |  | West Cheshire League | — | at Tadcaster Albion FC | |
| 07.Feb | Cheshire League |  | vs | | Yorkshire Amateur League | — | at Hyde United FC | |
| 14.Feb | Thames Valley Premier League |  | vs |  | Dorset Premier League | — | at Reading City FC | |
| 24.Jan | Kent County League |  | vs |  | Hertfordshire Senior County League | — | at Hollands & Blair FC | |
| 07.Feb | Essex & Suffolk Border League |  | vs |  | Southern Amateur League | — | at Harwich & Parkeston FC | |
| Quarter-Finals (by 7 March) | ||||||||
| Semi-Finals (by 9 April) | ||||||||
| Final (provisionally 9 May) | ||||||||
